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SUMMARY:A Label-free Microfluidic Assay to study drug diffusion through li
 pid membranes - Jehangir Cama
DTSTART:20140302T112000Z
DTEND:20140302T115000Z
UID:TALK51196@talks.cam.ac.uk
CONTACT:Trinity College Science Society
DESCRIPTION:The recent surge in numbers of antibiotic resistant bacteria h
 as stimulated interest in the mechanisms of antibiotic transport across ba
 cterial cell membranes. We present a label-free microfluidic assay that qu
 antifies the permeability coefficient of a broad spectrum fluoroquinolone 
 antibiotic\, norfloxacin\, as it diffuses across lipid membranes. We use g
 iant unilamellar vesicles as a model system\, tracking the diffusion of no
 rfloxacin molecules into the vesicles using the UV autofluorescence of the
  drug. We directly obtain the permeability coefficient without requiring k
 nowledge of the drug partition coefficient and validate theoretical predic
 tions for the effect of pH on norfloxacin permeability. This technique can
  be further extended to quantify the effect of nanopores embedded in the m
 embrane on drug diffusion.
LOCATION:Winstanley Lecture Theatre
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