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SUMMARY:Pattern recognition receptors: From the cell surface to endosomal 
 trafficking - Silke Robatzke\, The Sainsbury Laboratory\, Norwich Research
  Park
DTSTART:20140326T160000Z
DTEND:20140326T170000Z
UID:TALK51235@talks.cam.ac.uk
CONTACT:Helen Mawdsley
DESCRIPTION:Immune receptors constitute recognition sites to detect invadi
 ng pathogens and to trigger defenses\, and the abundance of these receptor
 s at the plasma membrane depends upon the dynamic membrane trafficking net
 work. Well-studied examples are the receptor kinases FLAGELLIN SENSING 2 (
 FLS2)\, EF-Tu RECEPTOR (EFR) from Arabidopsis and the Cf receptor-like pro
 teins from tomato. To understand the mode of actions of these immune recep
 tors\, we investigate their spatio-temporal dynamics using advanced micros
 copy approaches upon ligand-induced activation. I will describe the intern
 alization mechanism of FLS2 via a common\, clathrin-mediated endosomal pat
 hway of BAK1-dependent receptor kinases\, and I will discuss the role of k
 inase activity in this process. Focusing on late endosomal sorting\, I wil
 l present results showing that FLS2 is a likely cargo of the ESCRT machine
 ry and internalized inside multivesicular bodies for vacuolar degradation.
  These studies also suggest a role with regards to stomatal immunity and I
  will briefly describe our high-throughput imaging platform to investigate
  stomatal responses. To further study receptor-mediated endocytosis in pla
 nt immunity we investigated the spatio-temporal dynamics of tomato Cf-4\, 
 and I will describe recent results of its subcellular localization pattern
 s. Taken together\, advanced confocal imaging combined with functional stu
 dies allows us to tackle the dynamic changes involved in the interaction b
 etween plants and microbes at the cell surface. 
LOCATION:Auditorium of The Sainsbury Laboratory (Bateman Street)
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