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SUMMARY:From stripes to blood flow: cell fates of the lateral plate. - Pro
 fessor Christian Mosimann from the Institute of Molecular Life Sciences (I
 MLS)\, University of Zürich
DTSTART:20140501T133000Z
DTEND:20140501T143000Z
UID:TALK51367@talks.cam.ac.uk
CONTACT:Caroline Newnham
DESCRIPTION:The lateral mesoderm (LM) forms at the periphery of the develo
 ping vertebrate embryo and harbors the precursor cells for the cardiovascu
 lar and hematopoietic lineages\, the kidney\, and the limbs. How the LM in
 itially emerges during development and how it patterns into its diverse ce
 ll fates remain incompletely understood.  Deeper insight into the regulato
 ry complexes that drive initial LM formation will critically contribute to
  the in vitro differentiation of ES and iPS cells towards therapeutic cell
  types. Towards uncovering the primordial LM program\, we have been isolat
 ing and characterizing enhancer fragments that are active in the forming z
 ebrafish LM\, using both direct reporter and CreERT2 transgenics. Using ge
 netic lineage tracing\, we established that the draculin (drl) gene contai
 ns an early enhancer active from mid-gastrulation in the forming LM. At la
 ter stages\, two additional\, independent regulatory elements refine drl e
 xpression to the anterior heart field and the posterior blood and vessel l
 ineages. drl regulation thus provides a paradigm for the molecular interpl
 ay of LM and downstream cardiovascular/hematopoietic cell fates. We have e
 xploited the in vivo reporter features of drl to investigate the interplay
  of the developing heart lineages and the evolutionary origins of the card
 iac septation program. We are now combining genetic\, biochemical\, in tot
 o imaging\, and epigenetic approaches to investigate the upstream program 
 that drives activity of individual LM-specific regulatory elements.
LOCATION:Part II Room\, Department of Genetics
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