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SUMMARY:Second Harmonic Generation Imaging of voltage in dendritic spines.
  - Rafael Yuste\, Howard Hughes Medical Institute
DTSTART:20060712T150000Z
DTEND:20060712T160000Z
UID:TALK5147@talks.cam.ac.uk
CONTACT:Stefanie Reichelt
DESCRIPTION:Dendritic spines mediate most excitatory inputs in the brain. 
 Although it is clear that spines compartmentalize calcium\, it is still un
 known what role\, if any\, they play in integrating synaptic\ninputs. To i
 nvestigate the electrical function of spines directly we use second harmon
 ic generation (SHG) imaging of membrane potential in pyramidal neurons fro
 m hippocampal cultures and neocortical brain slices. With FM 4-64 as an in
 tracellular SHG chromophore\, we image membrane potential in the soma\, de
 ndritic branches and spines. The SHG response to voltage is linear and app
 ears based on an electro-optic mechanism. The SHG sensitivity of\nthe chro
 mophore in spines is similar to that of the parent dendritic shaft and the
  soma. Backpropagation of somatic action potentials generate SHG signals a
 t spines with similar amplitude and kinetics to somatic ones. Our data are
  the first optical measurements of membrane potential from spines and demo
 nstrate directly that backpropagating action potentials invade the spines.
 \n
LOCATION:Department of Biochemistry\, Sanger building\, Lecture theatre\, 
 Tennis Court Road
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