BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:Single Molecule Analysis Using Nanometer-Scale Pores - John J Kasi aonwicz DTSTART:20140901T150000Z DTEND:20140901T160000Z UID:TALK53780@talks.cam.ac.uk CONTACT:Dr. Ulrich Keyser DESCRIPTION:John J. Kasianowicz\, Ph.D.\nNIST\nPhysical Measurement Labora tory\nGaithersburg\, MD USA\n\nProtein nanometer-scale pores are the mole cular basis for nerve\, muscle and intercellular transport. Over the past two decades\, we've been exploring their potential for use in detecting\, identifying and physically characterizing individual molecules. Our resu lts suggest the method might prove useful for rapid DNA sequencing (1-5)\, sizing individual molecules in real-time (6-8)\, studying thermodynamic a nd kinetic proteins of single molecules (9)\, and screening for therapeuti c agents against lethal toxins (10).\n\n\n1) Kasianowicz\, J.J.\, et al. 1 996. PNAS (USA) 93\, 13770-13773\n\n2) Akeson\, M.\, et al. 1999. Biophys. J. 77\, 3227-3233\n\n3) Kasianowicz\, J.J.\, et al. 2008. Ann. Rev. Analy t. Chem. 1\, 737-766\n\n4) Kumar\, S.\, et al. 2012. Nature Scientific Re ports 2\, 684​\n\n5) Reiner\, J.E.\, et al. 2012. Chemical Reviews 112\ , 6431-6451\n\n6) Robertson\, J.W.F.\, et al. 2007. PNAS (USA) 104\, 820 7-8211\n\n7) Reiner\, J.E.\, et al. 2010. PNAS (USA) 107\, 12080-12085\n\ n8) Balijepalli\, A.\, et al. 2013. J. Am. Chem. Soc. 135\, 7064−7072\ n\n9) Reiner\, J.E.\, et al. 2013. J. Am. Chem. Soc. 135\, 3087-3094\n\n 10) Halverson\, K.M.\, et al. 2005. J. Biol. Chem. 280\, 34056–34062\n\n JOHN J. KASIANOWICZ (CV)\nDr. John Kasianowicz is the Leader of the Nanobi otechnology Project in the Physical Measurement\nLaboratory at NIST. He ea rned a Ph. D. in Physiology & Biophysics from the State University of New York at Stony Brook\, a M.A. in Physics from the State University of New Y ork at Stony Brook\, and a B.A. in Physics (with Distinction) from Boston University. John was a National Academy of Sciences/National Research Coun cil Research Associate in the Chemical Science and Technology Laboratory a t NIST prior to joining the staff and becoming a Leader of the Biomolecula r Materials Group. Currently\, John directs the research efforts of staff scientists\, post-doctoral fellows\, and graduate/undergraduate students.\ nDr. Kasianowicz pioneered research in four principal areas: 1) single mol ecule characterization\, quantification\, and identification\; 2) nanopore -based DNA sequencing\; 3) elucidating the mechanisms of bacterial toxin a ction\, and 4) development of new methods for membrane protein structure d etermination.\nHis seminal work in these fields opened other areas of inve stigation (e.g.\, nanopore-based single molecule force spectroscopy)\, new conferences dedicated to these subjects\, and NIH- and DARPA-based fundin g initiatives. Several companies (e.g.\, IBM\, Oxford Nanopore\, Illumina\ , Genia Technology\, Stratos\, Electronic BioSciences\, Quantum Biosystems \, and Roche) are pursuing John’s applied research to develop practical devices for the electronic detection and characterization of individual bi ological molecules\n(including DNA) with a prospect to construct an ubiqui tous and low-cost DNA sequencer. His current\nmajor focus is the developme nt of electronic systems to simultaneously quantify many biomarkers (prote ins\, DNA\, RNA\, etc.) in single cells\, tissue\, and blood. This researc h could have a marked impact on understanding basic cellular mechanisms an d aid the development of quantitative personalized medicine.\nJohn has pub lished 75 papers (Web of Science: 4751 citations\; the top 4 papers have 1 365\, 538\, 306\,\nand 223 citations each\, h-index 30\; Google Scholar: 6 231 citations\; the top 4 papers have 1798\, 706\, 383\,\nand 229 citation s each\, h-index 35\, i10 index 54). His most cited paper is in the top 19 cited publications (out of > 51\,000) at NIST/NBS since its inception in 1901\, including the work of 4 Nobel Laureates. He has given over 200 invi ted lectures\, seminars\, and contributed papers (including 4 keynote addr esses and\n24 plenary lectures) at conferences and institutions world-wide . John was the lead editor of the book “Structure and Dynamics of Confin ed Polymers” and he directed a NATO Advanced Research Workshop on Biolog ical\, Biophysical and Theoretical Aspects of Polymer Structure and Transp ort held in Hungary. He organized and co-organized several multidisciplina ry international meetings.\nDr. Kasianowicz's contributions have been reco gnized with several awards and honors. He was named a Fellow of the Americ an Physical Society’s Division of Biological Physics (2010) “for his p ioneering contributions to the field of biophysics including the detection \, identification\, characterization and quantification of biological and chemical polymers\, and for the development of a new method for protein st ructure determination”\, The Small Times “Best of Small Tech Researche r of the Year Finalist” (2007)\, and the Dept. of Commerce Silver Medal (2006) “for pioneering the use of nanopores to electronically probe the structure and function of single biomolecules and for the rapid detection of anthrax toxins”. His work has been highlighted in journals that addre ss a wide range of disciplines including Physics Today 65\, 29-31 (2012)\, Science 336\, 534 (2012) and 255\, 684 (1992)\, Nanomedicine 5\, 840 (201 0)\, Nature Physics 4\, 507 (2008)\; Analytical Chemistry (p. 4743\, July 2007)\; APS News 16\, 8 (February 2007)\; Science 311\, 1544-1546 (2006)\; The Biological Physicist (The Newsletter of the\nDivision of Biological P hysics of the American Physical Society) 1(5)\, 6-8 (2002)\; Analytical Ch emistry (p. 306A\, 1 June 2001)\, Chemical & Engineering News 79(19)\, 43 (7 May 2001)\; and Scientific American (September 1997). In addition\, he was recently selected as the Guest Editor of a special issue on “Ion Cha nnels in Disease” in Chemical Reviews. His teaching interests include ph ysics\, chemistry\, biology\, and biophysics. In addition\, he enjoys amat eur astronomy\, hiking\, golf\, and classical music. LOCATION:Pfizer Lecture Theatre\, Department of Chemistry\, Lensfield Rd\, CB2 1EW END:VEVENT END:VCALENDAR