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SUMMARY:Responses of mesenchymal stromal cells to hypoxia - Professor Rhod
 ri Ceredig\, National University of Ireland\, Galway
DTSTART:20141203T123000Z
DTEND:20141203T133000Z
UID:TALK54092@talks.cam.ac.uk
CONTACT:Sue Griffin
DESCRIPTION:Within the Regenerative Medicine Institute\, my laboratory is 
 interested in some of the basic biology of mesenchymal stem cells (MSC). \
 n\nMSC generally reside in a relatively hypoxic environment within the bon
 e marrow or in tumors. Enigmatically\, MSC are known to both support hemat
 opoiesis\, particularly early lymphopoiesis\, yet are also capable of inhi
 biting the function of mature lymphocytes. \n\nStarting with a collection 
 of non-transformed MSC lines\, we have shown that despite their prolonged 
 culture history\, they have retained physiological responsiveness to hypox
 ia. They are very resistant to DNA damage mediated by X-irradiation and ha
 ve an extremely efficient DNA damage response (DDR). Indeed\, recent data 
 indicates that hypoxia improves the DDR of MSC in a HIF-1-dependent fashio
 n 1. \n\nTo extend these studies\, we have begun analyzing the extracellul
 ar matrix (ECM) produced by these cells. Preliminary data indicates that E
 CM derived from MSC lines cultured in hypoxia increases transcripts for st
 em cell genes in MSC and has potent biological activity on MSC growth and 
 differentiation. \n\nWe are using this information to design culture syste
 ms to improve the clonal growth of mouse MSC freshly sorted from the bone 
 marrow. Some of the implications of these results will be discussed.\n\n
 
1 Sugrue T\, Lowndes NF and Ceredig R. Hypoxia enhances the radio-resis
 tance of mouse mesenchymal stromal cells. Stem Cells\, 2014 32:2188-200\n
LOCATION:Lecture Theatre\, Department of Pathology\, Tennis Court Road
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