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SUMMARY:Enhancing single-molecule detection with DNA origami nanostructure
 s - Prof. Dr. Philip Tinnefeld\, Institut für Physikalische &amp\; Theore
 tische Chemie TU Braunschweig
DTSTART:20141107T140000Z
DTEND:20141107T150000Z
UID:TALK55267@talks.cam.ac.uk
CONTACT:Dr Eileen Nugent
DESCRIPTION:In recent years\, we have combined DNA nanotechnology with sin
 gle-molecule\nfluorescence to create functional single-molecule devices su
 ch as nanoscopic\nrulers for superresolution microscopy and energy transfe
 r switches[1-4]. DNA\norigamis are also used to improve single-molecule de
 tection by fluorescence\nenhancement with nanoantennas or by single-molecu
 le placement in zeromode\nwaveguides using nanoadapters[5\, 6]. Especially
 \, fluorescence enhancement with\nself-assembled nanoparticles holds great
  potential for single-molecule\ndetection at higher concentrations but als
 o for diagnostic applications [7]. We\nwill discuss recent advancement in 
 fluorescence enhancement and how to\ndisentangle the complex factors that 
 influence the fluorescence of single\nmolecules near metallic nanostructur
 es[8\, 9]. \n\nReferences\n\n1.	J. J. Schmied\, M. Raab\, C. Forthmann\, E
 . Pibiri\, B. Wunsch\, T.\nDammeyer and P. Tinnefeld\, Nat Protoc\, 2014\,
  9\, 1367-1391.\n\n2.	J. J. Schmied\, C. Forthmann\, E. Pibiri\, B. Lalken
 s\, P. Nickels\, T.\nLiedl and P. Tinnefeld\, Nano Lett\, 2013\, 13\, 781-
 785.\n\n3.	J. J. Schmied\, A. Gietl\, P. Holzmeister\, C. Forthmann\, C.\n
 Steinhauer\, T. Dammeyer and P. Tinnefeld\, Nat Methods\, 2012\, 9\, 1133-
 1134.\n\n4.	I. H. Stein\, C. Steinhauer and P. Tinnefeld\, J Am Chem Soc\,
  2011\,\n133\, 4193-4195.\n\n5.	E. Pibiri\, P. Holzmeister\, B. Lalkens\, 
 G. P. Acuna and P. Tinnefeld\,\nNano Lett\, 2014\, 14\, 3499-3503.\n\n6.	G
 . P. Acuna\, F. M. Moller\, P. Holzmeister\, S. Beater\, B. Lalkens and\nP
 . Tinnefeld\, Science\, 2012\, 338\, 506-510.\n\n7.	P. Holzmeister\, G. P.
  Acuna\, D. Grohmann and P. Tinnefeld\, Chem Soc\nRev\, 2014\, 43\, 1014-1
 028.\n\n8.	J. V. Pellegrotti\, G. P. Acuna\, A. Puchkova\, P. Holzmeister\
 , A.\nGietl\, B. Lalkens\, F. D. Stefani and P. Tinnefeld\, Nano Lett\, 20
 14\, 14\,\n2831-2836.\n\n9.	P. Holzmeister\, E. Pibiri\, J. J. Schmied\, T
 . Sen\, P. Acuna Guillermo\nand P. Tinnefeld\, Nat Commun\, 2014\, accepte
 d.
LOCATION:Pippard Lecture Theatre\, Cavendish Laboratory\, Department of Ph
 ysics
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