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SUMMARY:Modelling DNA organisation by  DNA-binding proteins: the bridging-
 induced attraction and chromatin reconstitution - Davide Marenduzzo\, Univ
 ersity of Edinburgh
DTSTART:20150220T140000Z
DTEND:20150220T150000Z
UID:TALK56060@talks.cam.ac.uk
CONTACT:Gareth Conduit
DESCRIPTION:DNA in living cells of both bacteria and eukaryotes associates
  with proteins that continuously bind and dissociate. Some proteins affect
  local structure (such as histones and histone-like proteins)\, whereas ot
 hers act globally to compact whole chromosomal segments (such as CCCTC -bi
 nding factors).\n\nBrownian dynamics simulations are used here to model bo
 th DNA and DNA -bindin proteins. In the absence of any explicit interactio
 n between proteins\, or between templates\, we find that binding of protei
 n which are able to bridge the DNA\, spontaneously induces local DNA compa
 ction and protein aggregation. Small proteins interacting with naked DNA f
 orm into rows [as on binding of the bacterial histone-like nucleoid-struct
 uring protein (H-NS)]\, and large proteins into quasi-spherical aggregates
  (as on nanoparticle binding). We also explore how these ideas apply to th
 e self-assembly\, or reconstitution\, of chromatin\, which can be made in 
 vitro from DNA and histone octamers\, provided that appropriate experiment
 al protocols are followed accurately.
LOCATION:Pippard Lecture Theatre\, Cavendish Laboratory
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