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SUMMARY:Modelling DNA organisation by DNA-binding proteins: the bridging-i
 nduced attraction and chromatin reconstitution - Dr Davide Marenduzzo\, Le
 cturer in Biophysics\, University of Edinburgh
DTSTART:20150220T140000Z
DTEND:20150220T150000Z
UID:TALK56276@talks.cam.ac.uk
CONTACT:Dr Eileen Nugent
DESCRIPTION:DNA in living cells of both bacteria and eukaryotes associates
  with proteins that continuously bind and dissociate. Some proteins affect
  local structure (such as histones and  histone-like proteins)\, whereas o
 thers act globally to compact whole chromosomal segments (such as CCCTC-bi
 nding factors).\n\nBrownian dynamics simulations are used here to model bo
 th DNA and DNA-bindin proteins. In the absence of any explicit interaction
  between proteins\, or between templates\, we find that binding of protein
  which are able to bridge the DNA\, spontaneously induces local DNA compac
 tion and protein aggregation.\nSmall proteins interacting with naked DNA f
 orm into rows [as on binding of the bacterial histone-like nucleoid-struct
 uring protein (H-NS)]\, and large\nproteins into quasi-spherical aggregate
 s (as on nanoparticle binding).\nWe also explore how these ideas apply to 
 the self-assembly\, or reconstitution\, of chromatin\, which can be made i
 n vitro from DNA and histone octamers\, provided that appropriate experime
 ntal protocols are followed accurately.\n
LOCATION:Pippard Lecture Theatre\, Cavendish Laboratory
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