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SUMMARY:Quantitative high spatiotemporal resolution imaging of biological 
 processes - Dr Melike Lakadamyali\, NEST fellow\, ICFO\, Barcelona
DTSTART:20150227T140000Z
DTEND:20150227T150000Z
UID:TALK56277@talks.cam.ac.uk
CONTACT:Dr Eileen Nugent
DESCRIPTION:In the recent years\, super-resolution microscopy has emerged 
 as a powerful tool to image cells at the nanoscale\, giving important insi
 ghts into biological processes at the molecular level. These methods have 
 been so revolutionary that their impact has recently been recognized by th
 e Nobel Prize in Chemistry. While exciting developments over the last deca
 de have significantly advanced the capabilities of super-resolution micros
 copy\, important challenges remain in pushing its applications in biology.
  One limitation is the compromise between spatial and temporal resolution 
 that make these methods poorly suited to study dynamic processes. Another 
 challenge is our limited capability to extract quantitative information fr
 om the super-resolution images\, which is confounded by the photophysics o
 f the fluorophores. I will show how we are developing new approaches to ov
 ercome these challenges and demonstrate two novel biological applications 
 of super-resolution microscopy. In the first part of my talk\, I will summ
 arize our ongoing efforts to use a combination of single particle tracking
  and super-resolution microscopy to study how molecular motors such as dyn
 ein and kinesin overcome road blocks to transport their cargo. In the seco
 nd part of my talk\, I will show how we are using highly quantitative appr
 oaches to decipher the nanoscale organization of chromatin inside intact n
 uclei at the single cell level. Overall\, I will demonstrate that quantita
 tive\, high spatio-temporal resolution imaging holds great promise for sol
 ving important mysteries in biology. 
LOCATION:Pippard Lecture Theatre\, Cavendish Laboratory
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