BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Single cell transcriptomics. - Dr Steve Harvey\, Wellcome Trust Sa
 nger Institute\, Cambridge 
DTSTART:20150209T143000Z
DTEND:20150209T153000Z
UID:TALK56513@talks.cam.ac.uk
CONTACT:Caroline Newnham
DESCRIPTION:How a gene is transcribed in space and time is indicative of g
 ene function and regulation. While transcriptome studies have provided a d
 eep temporal analysis of gene expression during embryonic development most
  work has used pools of embryos. Therefore such analyses reflect transcrip
 tion from the sum of all embryonic parts\, subsequently masking the spatia
 l regulation of gene expression. I will discuss a single cell transcript c
 ounting method that we have developed and how we are using this to address
  cellular heterogeneity during embryonic development with the aim of impro
 ving our understanding of gene function. Using decreasing amounts in input
  RNA from whole embryos to small pools of cells and ultimately single cell
 s\, we have identified >100 genes that have previously not been described 
 to be spatially regulated during early stages of zebrafish gastrulation. B
 uilding on this topological map of spatially regulated genes\, we have ide
 ntified distinct populations of cells that exist in the zebrafish organise
 r. The experimental approach that I will describe will be applicable to ma
 ny different biological scenarios.
LOCATION:Part II Room\, Department of Genetics
END:VEVENT
END:VCALENDAR