BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Synthetic Probes for live-cell imaging - Kai Johnsson
DTSTART:20150311T161500Z
DTEND:20150311T180000Z
UID:TALK57171@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:The topic of my presentation will be how a combination of prot
 ein engineering and synthetic chemistry can be exploited to generate fluor
 escent and bioluminescent probes for live-cell imaging. \nIn the first par
 t of my presentation I will introduce a highly permeable and biocompatible
  near-infrared fluorophore that can be specifically coupled to intracellul
 ar proteins in live cells and tissues using different labeling techniques.
  The fluorogenic character of the probe and its high brightness permit liv
 e-cell imaging experiments without washing steps. The fluorophore is ideal
 ly suited for live-cell superresolution microscopy approaches using either
  stimulated emission depletion (STED) or stochastic single-molecule locali
 zation nanoscopy. The excellent spectroscopic properties of the probe comb
 ined with its ease of use in live-cell applications make it a powerful new
  tool for bioimaging.\nIn the second part of my talk\, I will talk about o
 ur attempts to introduce a new class of fluorescent sensor proteins that p
 ermit to visualize drug and metabolite concentrations in living cells with
  high spatial and temporal resolution. I will also discuss how these senso
 r proteins can be utilized for point-of-care therapeutic drug monitoring. 
 \n\nRelevant papers: \n\n1)	“Fluorogenic probes for live-cell imaging of
  the cytoskeleton” Lukinavicius\, G.\; Reymond\, L.\; D'Este\, E.\; Mash
 arina\, A.\; Gottfert\, F.\; Ta\, H.\; Guther\, A.\; Fournier\, M.\; Rizzo
 \, S.\; Waldmann\, H.\; Blaukopf\, C.\; Sommer\, C.\; Gerlich\, D. W.\; Ar
 ndt\, H. D.\; Hell\, S. W.\; Johnsson\, K. Nature Methods 2014\, 11\, 731 
 \n2) 	“Bioluminescent sensor proteins for point-of-care therapeutic drug
  monitoring” Griss\, R.\; Schena\, A.\; Reymond\, L.\; Patiny\, L.\; Wer
 ner\, D.\; Tinberg\, C. E.\; Baker\, D.\; Johnsson\, K. Nature Chem Biol 2
 014\, 10\, 598 \n3) 	“A near-infrared fluorophore for live-cell super-re
 solution microscopy of cellular proteins” Lukinavicius\, G.\; Umezawa\, 
 K.\; Olivier\, N.\; Honigmann\, A.\; Yang\, G.\; Plass\, T.\; Mueller\, V.
 \; Reymond\, L.\; Correa\, I. R.\, Jr.\; Luo\, Z. G.\; Schultz\, C.\; Lemk
 e\, E. A.\; Heppenstall\, P.\; Eggeling\, C.\; Manley\, S.\; Johnsson\, K.
  Nature Chemistry 2013\, 5\, 132\n
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
END:VEVENT
END:VCALENDAR