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SUMMARY:Fly meeting - Speaker to be confirmed
DTSTART:20150325T173000Z
DTEND:20150325T193000Z
UID:TALK58588@talks.cam.ac.uk
CONTACT:Saskia Suijkerbuijk
DESCRIPTION:*Benjamin Klapholz (Gurdon Institute)*\n\n_Talin mediates inte
 grin function: multiple molecular mechanisms for one general function_\n\n
 We used genetic and advanced imaging methods to show that talin \ncan adop
 t distinct\, tissue-specific\, mechanisms of action to mediate \nintegrin 
 function. Such diverse configurations may allow talin to sense \na variety
  of forces that affect cell-matrix adhesions.\n\n\n*Julian Ng and Ben Sutc
 liffe*\n\n_Genetically encoded probes for chemical labelling in Drosophila
 _\n\nFluorescent proteins (FP) and antibodies are powerful tools for\nanal
 ysing the structure of molecules and cells embedded within tissues.\nHowev
 er\, each method has drawbacks. FPs are readily detected when\nexpressed i
 n cells\, but they have weaker photon yields and limited\nspectral flexibi
 lity compared with organic dyes. Antibodies\, conjugated\nto small dyes\, 
 can highlight any endogenous protein. However their\nbulkiness limits the 
 labelling efficiency in thick tissue blocks during\nthe immunohistochemist
 ry process\, which can last for hours - days.\nFurthermore\, weakly intera
 cting antibodies can lead to off-target\neffects and high background stain
 ing.\nThis talk will highlight recently published [1] and unpublished work
  on\nchemical based tagging in Drosophila. We find that SNAP and Halo tag\
 nbased technologies can overcome some of the problems associated with FPs 
 and immunostaining\, as fluorescent chemical tagging leads to very fast\, 
 even\, high-signal and low-background labelling of thick tissues. We have 
 generated and validated in the brain a variety of UAS constructs along wit
 h two knockin lines (Brp\, Dlg) that should have applications in most fly 
 tissues.\n\n[1] see http://www.pnas.org/content/111/36/E3805.full and\njef
 ferislab.org/si/tagging\n
LOCATION:Gurdon Institute Tea Room
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