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SUMMARY:Interferometric scattering microscopy: From high-speed nanometry t
 o ultra-sensitive label-free imaging  - Professor Philipp Kukura\, Physica
 l and Theoretical Chemistry Laboratory\, University of Oxford 
DTSTART:20150424T130000Z
DTEND:20150424T140000Z
UID:TALK58941@talks.cam.ac.uk
CONTACT:Dr Eileen Nugent
DESCRIPTION:The primary goal of optical microscopy is to visualise micro o
 r even nanoscopic structure and dynamics. Developments over the past decad
 es have enabled routine studies down to the single molecule level and stru
 ctural observations far beyond the limits defined by the diffraction limit
  through the use of fluorescence as a contrast mechanism. Despite its many
  advantages\, fluorescence detection suffers from limitations such as the 
 need of introducing an appropriate label and a fundamentally limited photo
 n flux. I will highlight the capabilities of an alternative approach to op
 tical microscopy that relies on the detection of light scattering called i
 nterferometric scattering microscopy (iSCAT). I will illustrate how the un
 ique combination of nm precision and microsecond time resolution can be us
 ed to address fundamental questions about the processivity of molecular mo
 tors or the diffusion of receptors on living cells. In addition\, I will s
 how how iSCAT can detect\, image and track the motion of single proteins w
 ithout the need for any labels and how this sensitivity can be used to stu
 dy phenomena ranging from lipid nanodomains in model membranes to micelle 
 and vesicle dynamics relevant to pre-biotic chemistry and the origin of li
 fe.
LOCATION:Small Lecture Theatre\, Cavendish Laboratory
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