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SUMMARY:Modelling and characterization of amperometric enzyme biosensors -
  Peng Li
DTSTART:20061122T140000Z
DTEND:20061122T150000Z
UID:TALK5955@talks.cam.ac.uk
CONTACT:Dr. Richard\, Yong Qing Fu
DESCRIPTION:Abstract: Electrochemistry has been widely applied into the fi
 eld of biosensor in the past two decades.  Numerous novel sensors based on
  measuring electrochemical current\, capacitance and impedance have been s
 uccessfully developed.  They have been considered promising as next genera
 tion of biosensors due to their simplicity\, low cost and capability of in
 tegration\, over conventional technologies. In this work the homogeneous e
 nzymatic system has been systematically studied in the framework of cyclic
  voltammetry as a basis to develop amperometric biosensors based on the us
 e of enzyme as transducer.  A reversible electrode reaction with fast enzy
 me process is modeled and the differential equations are solved numericall
 y using finite element software.  Methods have been developed to extract k
 inetic parameters such as mediator reaction rate constant\, Michaelis-Ment
 en constant and so on.  The glucose / glucose oxidase / ferrocene monocarb
 oxylic acid system is applied as an example system to validate the results
 .  Good agreement has been achieved over a wide range of experimental cond
 itions and hence demonstrated that the method can be used to study general
  enzyme/mediator/substrate type of biosensor.
LOCATION:CAPE building\, seminar room 21-23. 9 JJ Thomson Ave. CB3 0FA
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