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SUMMARY:Discovering enhancer sequences with a role in vertebrate left-righ
 t patterning - Dennis Kostka (University of Pittsburgh)
DTSTART:20150715T140000Z
DTEND:20150715T150000Z
UID:TALK59957@talks.cam.ac.uk
CONTACT:Florian Markowetz
DESCRIPTION:Left-right (LR) symmetry breaking is a conserved developmental
  process\, and its first morphological manifestation is the looping of the
  heart in the early vertebrate embryo.  Therefore a better understanding o
 f the biology underlying LR-pattering is relevant in the context of congen
 ital heart disease and other cardiomyopathies. On the molecular level\, ti
 ght spatial and temporal control of gene expression is necessary for prope
 r LR-patterning to occur\, and enhancers are cis-regulatory DNA sequences 
 that play essential roles in gene regulation during development. To identi
 fy enhancer sequences with a role in LR-patterning\, we make use of genome
 -wide enhancer databases that cover hundreds of tissues and cell types to 
 define “enhancer candidates” based on sequence composition. Next\, we 
 use RNA sequencing data do identify LR-differentially expressed genes at t
 he time when heart looping first occurs. Finally\, we connect enhancer can
 didates with target genes\, and the most compelling findings will be the i
 nput for transgenic follow-up experiments in zebra fish and mouse. 
LOCATION:CRUK CI Room 013
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