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SUMMARY:&quot\;A subclass of neocortical fast spiking parvalbumin (FS-PV) 
 interneurons enwrapped by PeriNeuronalNet (PNN) expresses ADAMTS peptidase
 s. Possible roles of these metallo-peptidases in plasticity and critical p
 eriod.&quot\; - Dr Jean Rossier
DTSTART:20151007T110000Z
DTEND:20151007T120000Z
UID:TALK60416@talks.cam.ac.uk
CONTACT:Shannon Tinley-Browne
DESCRIPTION:The in situ hybridization Allen Mouse Brain Atlas was mined fo
 r proteases expressed in the somatosensory cerebral cortex. Among the 480 
 genes coding for protease/peptidases\, only 3 were found enriched in corti
 cal interneurons:  Adamts8 and Adamts15 belonging to the class of metzinci
 n proteases involved in reshaping the perineuronal net (PNN) and Mme encod
 ing for Neprilysin\, the enzyme degrading amyloid β−peptides. The patte
 rn of expression of metalloproteases (MP) was analyzed by single cell RT m
 ultiplex PCR after patch-clamp and was compared with the expression of 10 
 canonical interneurons markers and 12 additional genes from the Allen Atla
 s. Clustering of these genes by K-means algorithm displays five distinct c
 lusters. Among these 5 clusters\, two fast spiking (FS) interneurons clust
 ers expressing the calcium-binding protein Pvalb were identified\, one co-
 expressing Pvalb with Sst (PV-Sst) and another co-expressing Pvalb with th
 ree metallopeptidases Adamts8\, Adamts15 and Mme (PV-MP). PV-MP interneuro
 ns were found surrounded by PNN while the one expressing Sst\, PV-Sst were
  not. These observations will be discussed in view of current hypothesis o
 n brain plasticity\, critical period and long term memory . 
LOCATION:Brain Repair Centre\, Forvie Site\, Robinson Way
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