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SUMMARY:Continuous Droplet Interface Crossing Encapsulation (cDICE): artif
 icial cells and capsules - Dr. Gladys Massiera.  Laboratoire Charles Coulo
 mb (L2C – UMR 5221)\, CNRS - Université de Montpellier\, Place E. Batai
 llon\, Montpellier\, France
DTSTART:20151023T130000Z
DTEND:20151023T140000Z
UID:TALK61086@talks.cam.ac.uk
CONTACT:Dr. Hernandez-Ainsa
DESCRIPTION:The Continuous Droplet Interface Crossing Encapsulation (cDICE
 ) is an easy and robust method for producing\, at high yield\, monodispers
 e lipid vesicles with a controlled content as well as capsules with a desi
 gned shell. We will discuss the physical mechanisms involved in the produc
 tion of both cDICE vesicles and capsules\, and several applications\, such
  as the production of artificial red blood cell or biolarvicide capsules.\
 n\nThe set-up consists in a cylindrical rotating topped-chamber\, filled w
 ith a Dispersing Aqueous Solution (DAS) and a lower density solution (LDS)
  that form a vertical interface due to the centrifugal force. Droplets of 
 the aqueous solution to be encapsulated (EAS) are injected in the LDS by d
 ripping. Centrifugation of these droplets leads to their crossing of the L
 DS/DAS interface either at low or high inertia depending on the rotation s
 peed. Low inertia is the regime used to produce vesicles: during the dropl
 ets ‘flight’ across the LDS layer\, a monolayer of lipids dispersed in
  the LDS adsorbs onto the aqueous droplets\, which zip with the monolayer 
 spontaneously formed at the LDS/DAS interface\, leading to vesicles suspen
 ded in the DAS. \nFor the design of original capsules\, the droplets cross
  the interface at high inertia\, thereby entraining some LDS\, which will 
 in fine constitute the shell of the capsules stabilized by liquid to solid
  temperature controlled transition or UV polymerization.\n The cDICE metho
 d allows to encapsulate various biological solutions (biopolymers\, hemogl
 obin\, colloids\, polymeric gels\, cells…) in membranes that can be comp
 osite and/or assymetric\, or polymeric. Its key-steps will be discussed in
  terms of hydrodynamical and colloidal interactions.\n
LOCATION:Small Lecture Theatre\, Cavendish Laboratory
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