BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:A pioneering imaging approach to arbuscule development in rice - R
 onelle Roth\, Uta Paszkowski Lab
DTSTART:20151119T130000Z
DTEND:20151119T140000Z
UID:TALK61179@talks.cam.ac.uk
CONTACT:38889
DESCRIPTION:Within the roots of most land plants beneficial arbuscular myc
 orrhiza fungi (AMF) form complex tree-shaped feeding structures called arb
 uscules.  Monumental cellular re-differentiation and reprogramming in the 
 inner root cortex result in the de novo synthesis of a host-derived membra
 ne that surrounds the arbuscule\, the peri-arbuscular membrane (PAM). This
  functional symbiosome interface facilitates nutrient exchange between fun
 gus and plant.  The extent to which fungal and host membranes are tailored
  for this plant-fungal dialogue and our understanding of how different PAM
 -specific proteins are retained within the PAM remain unknown.  This is pa
 rtly due to the low resolution of live-cell imaging of inner cortical rice
  root cell layers using conventional confocal laser scanning microscopy (C
 LSM).  The aim of this project is to 1.) develop innovative imaging approa
 ches to investigate ultra-structural modifications to plant and fungal mem
 branes during arbuscule differentiation and 2.) to pioneer high resolution
  live-cell bioimaging to investigate dynamic changes to PAM-specific prote
 ins during arbuscule development.  High pressure frozen (HPF) Transmission
  Electron Microscopy (TEM) of rice roots colonized by Rhizophagus irregula
 ris has uncovered exciting fungal membrane structures that resemble Extrac
 ellular Vesicles (EVs) at the AMF-plant interface that are capable of pass
 age through the fungal cell wall into the peri-arbuscular space.  Multi-ph
 oton confocal microscopy (MPCM) was developed for rice roots to allow deep
 -tissue time-lapse imaging of fluorescently-tagged PAM proteins during arb
 uscule development.  Results indicate that separate spatio-temporal mechan
 isms control trafficking of functionally diverse PAM proteins during arbus
 ucle development.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
END:VEVENT
END:VCALENDAR