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SUMMARY:Discovery of methylation loci and analyses of differential methyla
 tion in Dicer-like mutants of Arabidopsis - Thomas Hardcastle\, David Baul
 combe Lab
DTSTART:20151203T130000Z
DTEND:20151203T133000Z
UID:TALK61181@talks.cam.ac.uk
CONTACT:38889
DESCRIPTION:Cytosine methylation arises from the addition of a methyl grou
 p to a cytosine's C5 carbon residue. Cytosine methylation is widespread in
  most eukaryotic genomes and is known to play a substantial role in variou
 s regulatory pathways. Unmethylated cytosines may be converted to uracil t
 hrough the addition of sodium bisulphite\, allowing genome-wide quantifica
 tion of cytosine methylation via high-throughput sequencing. The data thus
  acquired allows the discovery of methylation 'loci'\, contiguous regions 
 of methylation consistently methylated across biological replicates. The m
 apping of these loci allows for associations with other genomic factors to
  be identified\, and for analyses of differential methylation to take plac
 e. \nA statistical model is developed that accounts for biological replica
 tion and variable rates of non-conversion of cytosines in each sample to c
 ompute posterior likelihoods of methylation at each locus within an empiri
 cal Bayesian framework. The same model is used as a basis for analysis of 
 differential methylation between multiple experimental conditions. These a
 nalyses are demonstrated on a set of data derived from Dicer-like mutants 
 in Arabidopsis to reveal complex interactions between the different Dicer-
 like mutants and their methylation pathways.\n
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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