BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Nanophotonic approaches to investigate the spatiotemporal organiza
 tion of biological membranes - Maria F. Garcia-Parajo\, ICFO-Institute of 
 Photonic Sciences\, Castelldefels (Barcelona)\, Spain and ICREA- Instituci
 ó Catalana de Recerca i Estudis Avançats\, Barcelona\, Spain
DTSTART:20151120T140000Z
DTEND:20151120T150000Z
UID:TALK61365@talks.cam.ac.uk
CONTACT:Dr. Hernandez-Ainsa
DESCRIPTION:A hot topic in cell biology is to understand the specific nano
 meter-scale organization and distribution of the surface machinery of livi
 ng cells and its role regulating the spatiotemporal control of different c
 ellular processes. Cell adhesion\, pathogen recognition or lipid-mediated 
 signaling\, all fundamentally important processes in immunology\, are gove
 rned by molecular interactions occurring at the nanoscale. From the techni
 cal point of view\, the quest for optical imaging of biological processes 
 at the nanoscale has driven in recent years a swift development of a large
  number of microscopy techniques based on far-field optics. These super-re
 solution methods are providing new capabilities for probing biology at the
  nanoscale by fluorescence. While these techniques conveniently use lens-b
 ased microscopy\, the attainable resolution and/or localization precision 
 severely depend on the sample fluorescence properties. True nanoscale opti
 cal resolution free from these constrains can alternatively be obtained by
  interacting with fluorophores in the near-field. Indeed\, near-field scan
 ning optical microscopy (NSOM) using subwavelength aperture probes is one 
 of the earliest approaches sought to achieve nanometric optical resolution
  [1]. More recently\, photonic antennas have emerged as excellent alternat
 ive candidates to further improve the resolution of NSOM by amplifying ele
 ctromagnetic fields into regions of space much smaller than the wavelength
  of light [2]. In this talk I will describe our efforts towards the fabric
 ation of different nanoantenna probe configurations as well as 2D antenna 
 arrays for applications in nano-imaging and spectroscopy of living cells [
 3-5]. For nanoscale imaging\, we have recently pushed the limits of spatia
 l resolution by demonstrating dual colour imaging of individual fluorescen
 t molecules with true 20nm spatial resolution and sub-nanometre localizati
 on accuracy using antenna probes [4]. In parallel\, we have recently demon
 strated that photonic antennas allow the recording of individual lipid dif
 fusion on living cell membranes in regions as small as 20nm in size [5]. F
 inally\, I will summarize our efforts towards dual–colour fluorescent cr
 oss correlation spectroscopy in living cells with a spatial confinement of
  50nm. \n\nReferences:\n\n[1]	P. Hinterdorfer\, M.F. Garcia-Parajo\, Y. Du
 frene\,  “Single-molecule imaging of cell surfaces using near-field nano
 scopy”\, Acc. Chem. Res. 45\, 327-336\, 2012.\n[2]	M.F. Garcia-Parajo\, 
 “Photonic antennas focus in on biology”. Nature Photonics\, 2\, 201-20
 4\, 2008.\n[3]	D. Punj\, M. Mivelle\, S. B. Moparthi\, T. S. van Zanten\, 
 H. Rigneault\, N. F. van Hulst\, M. F. García-Parajó\, J. Wenger\, “A 
 plasmonic ‘antenna-in-box’ platform for enhanced single-molecule analy
 sis at micromolar concentrations”\, Nature Nanotechnol. 8\, 512-516\, 20
 13.\n[4]	M. Mivelle\, T. S. van Zanten\, M. F. Garcia-Parajo\, “Hybrid p
 hotonic antennas for subnanometer multicolor localization and nanoimaging 
 of single molecules”\, Nano Lett. 4\, 4895-4900\, 2014.\n[5]	V. Flauraud
 \, T. S. van Zanten\, M. Mivelle\, C. Manzo\, M. F. Garcia Parajo\, J. Bru
 gger\, “Large-scale arrays of bowtie nanoaperture antennas for nanoscale
  dynamics in living cell membranes”\, Nano Lett. 15\, 4176-4182 2015.\n
LOCATION:Small Lecture Theatre\, Cavendish Laboratory
END:VEVENT
END:VCALENDAR