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SUMMARY:Mechanical coupling between endoderm invagination and axis extensi
 on in Drosophila - Claire Lye\, Sanson lab
DTSTART:20151028T173000Z
DTEND:20151028T193000Z
UID:TALK62006@talks.cam.ac.uk
CONTACT:Clara Sidor
DESCRIPTION:*How genetic programs generate cell-intrinsic forces to shape 
 embryos is\nactively\nstudied\, but less so how tissue-scale physical forc
 es impact\nmorphogenesis.  I will\ndiscuss the role of tissue-scale forces
  during Drosophila germband\nextension (GBE).\nPrevious work in our lab ha
 d shown that cells elongate in the\nanteroposterior (AP)\naxis in the exte
 nding germband\, suggesting that an extrinsic tensile force\ncontributed t
 o body axis extension.  {Butler LC et al. 2009. Nat Cell Biol\n11:\n859-86
 4}.\n\nTo identify the morphogenetic movements that could be the source of
  this\nextrinsic\nforce\, we mapped gastrulation movements temporally usin
 g light sheet\nmicroscopy to\nimage whole Drosophila embryos. We found tha
 t both mesoderm and endoderm\ninvaginations are synchronous with the onset
  of GBE. AP cell elongation\nremains when\nmesoderm invagination is blocke
 d\, but is abolished in the absence of endoderm\ninvagination. This sugges
 ted that endoderm invagination is the source of\nthe tensile\nforce.\n\nWe
  next looked for evidence of this force in acellular embryos\, which lack\
 npolarized\ncell intercalation but in which endoderm invagination still oc
 curs. We\nused laser\nablation to show that tension is increased in the AP
  orientation compared\nto DV\norientation in the posterior germband of the
 se mutants.  We propose that\nendoderm\ninvagination is the source of the 
 extrinsic force contributing to germband\nextension. This highlights the i
 mportance of physical interactions between\ntissues\nduring morphogenesis.
 *\n
LOCATION:Gurdon Institute Tea Room
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