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SUMMARY:Proteomics in cell signaling and cancer research - Matthias Mann\,
  Max-Planck Institute of Biochemistry\, Munich and Novo Nordisk Foundation
  Center for Protein Research\, Copenhagen
DTSTART:20160121T130000Z
DTEND:20160121T140000Z
UID:TALK62351@talks.cam.ac.uk
CONTACT:Kate Davenport
DESCRIPTION:Mass spectrometry based proteomics has advanced tremendously i
 n the last few years. We describe a shotgun proteomics workflow that allow
 s us to detect and quantify the large majority of the proteins expressed i
 n a biological system such as cancer cell lines and even formalin-fixed\, 
 paraffin embedded material. This included streamlined and highly efficient
  sample preparation\, analysis with very high sequencing speed using moder
 n mass spectrometers and bioinformatic analysis using the MaxQuant and Per
 seus platforms. Efforts in our group have focused on ‘single shot’ ana
 lysis and we demonstrate very high coverage in this mode (Mann et al.\, Mo
 l. Cell\, 2013). We have also extended this concept to the analysis of int
 eractomes (Hein et al. Cell 2015) and phosphorylation\, where the ‘EasyP
 hos’ method now allows acquiring large numbers of phosphoproteomes\, for
  instance for the analysis of in vivo signaling (Humphrey et al. Nat. Biot
 ech\, 2015). Together such developments make proteomics increasingly relev
 ant to translational research as I will discuss in this talk. 
LOCATION:CRUK CI Lecture Theatre
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