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SUMMARY:Single cell RNA-sequencing reveals a continuous spectrum of differ
 entiation in haematopoietic cells. - Dr Ana Cvejic\, Department of Haemato
 logy\, University of Cambridge
DTSTART:20160128T140000Z
DTEND:20160128T150000Z
UID:TALK62554@talks.cam.ac.uk
CONTACT:Caroline Newnham
DESCRIPTION:The transcriptional programs that govern haematopoiesis have m
 ainly been investigated by population-level analysis of haematopoietic ste
 m and progenitor cells\, which cannot reveal the continuous nature of the 
 differentiation process. Here we applied single cell RNA-sequencing to a p
 opulation of haematopoietic cells in zebrafish as they undergo thrombocyte
  lineage commitment. By reconstructing their developmental chronology comp
 utationally\, we were able to place each cell along a continuum from stem 
 cell to mature cell\, refining the traditional lineage tree. The progressi
 on of cells along this continuum is characterised by a highly coordinated 
 transcriptional program\, displaying simultaneous suppression of genes inv
 olved in cell proliferation and ribosomal biogenesis as the expression of 
 lineage specific genes increases. Within this program\, there is substanti
 al heterogeneity in the expression of the key lineage regulators. Overall\
 , the total number of genes expressed\, as well as the total mRNA content 
 of the cell\, decreases as the cells undergo lineage commitment.
LOCATION:Part II Room\, Department of Genetics
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