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SUMMARY:Shining new light on chemistry and biology with inteferometric sca
 ttering microscopy - Professor Philipp Kukura ( Dept of Chemistry\, Univer
 sity of Oxford )
DTSTART:20151208T160000Z
DTEND:20151208T170000Z
UID:TALK62827@talks.cam.ac.uk
CONTACT:Alice Wood
DESCRIPTION:The fundamental goal of optical microscopy is to visualise and
  thereby enable the study of dynamics on the microscopic or even nanoscopi
 c scale. The past decades have been dominated by fluorescence-based approa
 ches\, in particular in super-resolution methodologies. Despite the advant
 ages of fluorescence imaging\, the requirement of introducing labels can b
 e both complex and perturbative\, while limited photophysics and photochem
 istry limits imaging speed\, precision and duration. I will highlight the 
 capabilities of an alternative approach to optical microscopy based on lig
 ht scattering called interferometric scattering microscopy. Contrary to in
 tuition\, I will show that iSCAT can achieve sensitivities approaching and
  possibly rivalling those of a fluorescence microscope including label-fre
 e imaging of single molecules. Importantly\, this sensitivity has wide-ran
 ging applications for studies of nanoscale phenomena in general\, such as 
 phase separation\, dynamics at interfaces\, bilayers or biological filamen
 ts.
LOCATION:Pfizer LT
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