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SUMMARY:Live cell biochemistry by light - Alessandro Esposito (Hutchison/M
 edical Research Council Research Centre)
DTSTART:20160219T150000Z
DTEND:20160219T160000Z
UID:TALK64223@talks.cam.ac.uk
CONTACT:Stefanie Reichelt
DESCRIPTION:Many forms of cancer begin with oncogenic events\, which\, in 
 normal cells\, elicit growth cessation or cell death rather than transform
 ation\, due to the activation of tumour suppressive networks. We are inter
 ested in dissecting the signaling pathways that lead from oncogenic events
  to tumour suppressive mechanisms\, and in particular\, how cells integrat
 e extracellular and intracellular signals to preserve tissue-\, cell- and 
 genome homeostasis in the face of oncogenic challenge.\n\nHowever\, we rec
 ognize that state of the art technologies do not permit to characterize a 
 number of biochemical reactions in the living cells thereby limiting our c
 apabilities to establish causal dependencies between the spatiotemporal dy
 namics of biochemical networks and cellular decisions. Therefore\, we devo
 ted significant efforts to develop novel fluorescence-based assays with th
 e aim to enable complex biochemical measurements in single living cells. \
 n\nIn this talk\, I will review state-of-the-art biochemical imaging techn
 iques (e.g.\, translocation sensors for phosphorylation\, FRET-based senso
 rs and FLIM) and techniques for the light-dependent induction of biochemic
 al events. Furthermore\, I will demonstrate our latest developments of fas
 t non-invasive techniques to simultaneously measure several biochemical re
 actions within the living cells with high spatio-temporal resolution and t
 he use of optogenetics in cell biology and cancer biology (e.g.\, fast act
 ivation of oncogenic signalling) in particular.\n\nThe possibility to inte
 rrogate signal transduction pathways non-invasively with the highest spati
 otemporal and biochemical resolutions will enable us to elucidate mechanis
 ms underlying oncogenesis and their cellular heterogeneity. \n
LOCATION:CRUK Cambridge Institute Lecture Theatre
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