BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:A novel biophysical method for the study of tethered signalling re
 actions - Omer Dushek (University of Oxford)
DTSTART:20160217T150000Z
DTEND:20160217T160000Z
UID:TALK64717@talks.cam.ac.uk
CONTACT:INI IT
DESCRIPTION:Many signalling reactions depend on the signalling protein fir
 st localising near its substrate before catalysing a reaction. A common ex
 ample is the binding of cytosolic enzymes to the unstructured cytoplasmic 
 tails of immune receptors. The tethering (binding) of these enzymes to the
  tails of immune receptors influences the local concentration of substrate
  that they experience. In contrast to cytosolic reactions\, we currently d
 o not have experimental tools to study tethered signalling reactions. In t
 his work-in-progress talk\, I will present an experimental assay that we h
 ave been using to study tethered signalling. I will show that the PDE mode
 l fails to fit the data (which is averaged over moles of protein) whereas 
 the equivalent stochastic simulation can fit the data. We derived a modifi
 ed PDE model based on a pair density formalism that can fit the data and t
 he stochastic simulation. Ultimately\, we are able to recover 4 parameters
  that characterise a tethered signalling reaction from the data: binding r
 ates\, catalytic rate\, and a parameter that determines the properties of 
 the tether.
LOCATION:Seminar Room 2\, Newton Institute
END:VEVENT
END:VCALENDAR