BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Plasmonic nanobiosensors: From therapeutic drug monitoring to opto
 physiology of live cells   - Prof. Jean-François Masson\, Université de 
 Montréal
DTSTART:20160301T140000Z
DTEND:20160301T150000Z
UID:TALK64789@talks.cam.ac.uk
CONTACT:Sharon Connor
DESCRIPTION:In this presentation\, the properties of different plasmonic n
 anomaterials will be discussed in the context of clinical and biological s
 ensing. In our research\, we have studied and integrated novel plasmonic m
 aterials based on nanoparticle and hole arrays\, surface chemistry relying
  on peptide monolayers and unique instrumental designs for sensing biomole
 cules of importance in disease detection\, monitoring the course of treatm
 ent of patients during ongoing therapies or of general interest in biologi
 cal systems. The plasmonic properties of nanohole and microhole arrays wer
 e studied in different excitation modes (transmission and attenuated total
  reflection) for plasmonic sensing and in surface-enhanced excitation. The
  nanohole and microhole were arrayed using photolithography on a 4” wafe
 r and the nanohole arrays were integrated in a 96-well plate reader. This 
 platform was used to screen antibodies for PSA sensing and to monitor meth
 otrexate\, an anticancer agent used in chemotherapy. We have also develope
 d a SPR and LSPR sensing platform based on a small and portable instrument
 . Competition assays were validated for therapeutic drug quantitation\, su
 ch as methotrexate and antibiotics and for monitoring therapeutic response
 s of patients undergoing leukemia treatments. Lastly\, we are currently ex
 ploring the concept of optophysiology using plasmonic nanopipettes for mon
 itoring live cell secretion events. Due to the absence of general techniqu
 es for detecting metabolites near live cells\, developing tool to monitor 
 cell secretion events remains a challenge to overcome in chemical analysis
 . Plasmonic nanopipettes were developed based on the decoration of patch c
 lamp nanocapillaries with Au nanoparticles. The plasmonic nanopipette is t
 hus competent for dynamic SERS measurements in the liquid environment near
  cells. This nanobiosensor was tested with the detection of small metaboli
 tes near live MDCKII cells.
LOCATION:Pfizer Lecture Theatre\,  Department of Chemistry
END:VEVENT
END:VCALENDAR