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SUMMARY:In vivo analysis of calcium dynamics in Arabidopsis: tools and app
 lications - Alex Costa\, University of Milan
DTSTART:20160519T120000Z
DTEND:20160519T130000Z
UID:TALK65484@talks.cam.ac.uk
CONTACT:38889
DESCRIPTION:In plants\, rises in cytosolic Ca2+ concentration ([Ca2+]cyt) 
 occur in response to both biotic and abiotic stimuli. These rises can\, de
 pending on the stimulus\, display the form of a single transient or repeti
 tive Ca2+ oscillations and are commonly designated as “Ca2+ signatures
 ”. Generation and shaping of [Ca2+]cyt signatures depends on fine-tuning
  of Ca2+ influxes and effluxes occurring at both the plasma membrane (PM) 
 and membranes of the different subcellular compartments. The opening of Ca
 2+-permeable influx channels in response to a stimulus will release Ca2+ i
 nto the cytosol and cause the generation of a Ca2+ spike\, while activity 
 of Ca2+ efflux transporters (H+-Ca2+ antiporters and Ca2+-ATPases) will re
 turn the [Ca2+]cyt to resting concentrations. In order to understand how t
 he cytosolic Ca2+ dynamics are generated and shaped in different cell type
 s\, two issues need to be addressed: i) the study of how do organelles par
 ticipate in these processes and ii) the possibility to perform single cell
  Ca2+ analyses in complex tissues and organs\, in natural context and in c
 lose-to physiological conditions.\n\nThe seminar will present data about: 
 \n\na)	the in planta use of the genetically encoded FRET (Förster Resonan
 ce Energy Transfer)-based Ca2+ Cameleon sensor. Attention will be given to
  the developed Cameleon probes for the analyses of Ca2+ dynamics in severa
 l subcellular compartments\; \n\nb)	development of a new microscopy soluti
 on\, based on Selective Plane Illumination Microscopy (SPIM)\, for FRET-ba
 sed Ca2+ imaging analyses in Arabidopsis root cells\;\n\nc)	use of the pre
 sented technologies for the study of channels and pumps involved in the ge
 neration and regulation of cytosolic Ca2+ dynamics. \n
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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