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SUMMARY:Approaching the physical limits of high-resolution electron cryomi
 croscopy - Dr. Christopher J. Russo\, MRC Laboratory for Molecular Biology
 \, U.K.
DTSTART:20160406T103000Z
DTEND:20160406T113000Z
UID:TALK65505@talks.cam.ac.uk
CONTACT:Dr Christopher John Edgcombe
DESCRIPTION:Despite recent advances in electron cryomicroscopy (cryo-EM) a
 nd the atomic resolution afforded by modern aberration corrected electron 
 optics\, the structures of many proteins and macromolecular complexes cann
 ot be determined by cryo-EM because the individual protein molecules move 
 during electron irradiation. This blurs the images so they cannot be align
 ed with each other to calculate a 3D density map. I will discuss the types
  of physical movement at various length scales that occur in biological sp
 ecimen during high-energy electron irradiation\, and show how reducing thi
 s movement leads to improved micrographs and density maps. Our approach to
  the problem combines detailed study of the physical origins of specimen m
 ovement with re-engineering the specimen support to reduce this movement t
 o below the resolution limit of the microscope. Further\, I will discuss p
 rotein-surface interactions in the context of electron microscopy\, which 
 are critical to the successful determination of protein structures. The st
 udy\, and ultimately the control of\nprotein interactions with tailored su
 rfaces designed for cryo-EM\, may represent an important opportunity for t
 he use of a near-field microscope.\n
LOCATION:Centre for Mathematical Sciences\, meeting room MR2
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