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SUMMARY:Guiding Principles of Selective Autophagy in Plants - Dr Yasin Dag
 das\, Sainsbury Lab\, Norwich 
DTSTART:20160427T100000Z
DTEND:20160427T110000Z
UID:TALK65980@talks.cam.ac.uk
CONTACT:david baulcombe
DESCRIPTION:Autophagy is a multifaceted membrane trafficking pathway invol
 ved in adaptation to cellular stress conditions. Activation of autophagy l
 eads to formation of double membrane vesicles called autophagasomes\, whic
 h carry autophagic cargo to the vacuole for recycling. Autophagosomes are 
 marked by a ubiquitin like protein called ATG8. It is now well established
  that rather than being a bulk degradation process autophagy is highly sel
 ective. Selective autophagy is mediated by cargo receptors that interact w
 ith ATG8 via a conserved ATG8 Interacting Motif (AIM) and recruit autophag
 ic cargo to the autophagosomes. The molecular basis of autophagic cargo re
 cruitment and biological roles of selective autophagy are poorly understoo
 d. Recently\, we discovered that a secreted effector of the Irish potato f
 amine pathogen\, named PexRD54\, binds to the autophagy marker protein ATG
 8 via an AIM. PexRD54 does not have a negative effect on autophagic flux a
 nd stimulates autophagosome formation. To investigate the biological funct
 ion of PexRD54\, we studied the plant autophagy receptor Joka2\, which als
 o interacts with ATG8. Overexpression of Joka2 in planta limited pathogen 
 infection\, suggesting a role for Joka2/ATG8 selective autophagy in respon
 se to oomycete infection. Remarkably PexRD54 was able to out-compete Joka2
  for binding to ATG8 and restore full pathogen virulence. Our findings poi
 nt to a model in which an effector from the Irish potato famine pathogen a
 ntagonizes plant autophagy receptor to enhance pathogen virulence. Using P
 exRD54 as a molecular probe\, we developed a model which may explain the m
 olecular basis of selective autophagy. I will present our recent findings 
 that validate our model and explain how we will exploit this model to diss
 ect the biological roles of selective autophagy in plants. 
LOCATION:Large Lecture Theatre\, Department of Plant Sciences
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