BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:NEW ADVANCES IN ISOTHERMAL TITRATION CALORIMETRY: GOING FAR BEYOND
  TRADITIONAL ANALYSIS WITH AFFINIMETER - AFFINImeter
DTSTART:20160516T130000Z
DTEND:20160516T140000Z
UID:TALK66227@talks.cam.ac.uk
CONTACT:Alice Wood
DESCRIPTION:Isothermal Titration Calorimetry (ITC) is a physical technique
 \, essential in the field of molecular recognition. It is widely used in a
 cademic research laboratories and also in the pharmaceutical industry wher
 e it is used right through the initial steps of hit validation and lead op
 timization of drug discovery programs1.\n\nTraditionally\, ITC offers a di
 rect and quantitative measure of the binding affinity (KA)\, enthalpy (ΔH
 ) and stoichiometry and for this it has been long considered the gold stan
 dard for quantitative measurements of biomolecular interactions. But the b
 attery of information potentially available from ITC goes far beyond the t
 hermodynamic profiling of binding events. A proper analysis of ITC data re
 gistered under optimized experimental conditions can yield rich thermodyna
 mic and structural information that contributes to the elucidation of stru
 cture activity relationships (SAR) and mechanistic aspects of interactions
 . Besides\, the kinetic profiling can be readily determined from standard 
 ITC thermograms and the method KinITC\, as these are a measure of the heat
  change occurred upon titrant injection over time2. Until recently\, the m
 ain hurdles to exploit the full potential of ITC was not in the experiment
 al facet but in the data analysis step.\n \nHerein we bring to the fore th
 at the combination of technical improvements in ITC equipment\, together w
 ith novel analytical tools like those incorporated in the software AFFINIm
 eter\, is now letting to exploit the full potential of the technique. This
  is illustrated with selected examples of ITC analyses of challenging liga
 nd-receptor interactions of interest.\n\n\nReferences \n1.	Klebe G. (2015)
 . Applying thermodynamic profiling in lead finding and optimization. Natur
 e Reviews Drug Discovery\, 14(2)\, pp.95-110.\n2.	(a) Dumas P.\, Ennifar E
 .\, Da Veiga C.\, Bec G.\, Palau W.\, Di Primo C.\, Piñeiro A.\, Sabin J.
 \, Muñoz E.\, Rial J. (2016). Extending ITC to kinetics with kinITC. Meth
 ods Enzymol (567)\, pp.157-180. (b) Dumas P.\, Ennifar E.\, Bec G.\, Piñe
 iro A.\, Sabin J.\, Muñoz E.\, Rial J. (2016). Malvern application note. 
 Implementation of kinITC into AFFINImeter\n
LOCATION:Todd Hamied\, Department of Chemistry
END:VEVENT
END:VCALENDAR