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SUMMARY:On the development and calibration of an in vitro platform to inve
 stigate the response of stem cells over a wide range of pressure and strai
 n rate - David Sory\, Royal British Legion Centre for Blast Injury Studies
 \, Imperial College London
DTSTART:20161027T150000Z
DTEND:20161027T160000Z
UID:TALK66398@talks.cam.ac.uk
CONTACT:Stephen Walley
DESCRIPTION:Increased interest in post-traumatic effects associated with b
 last wave exposure\, such as traumatic brain injury and heterotopic ossifi
 cation has led to more interest into how cells respond to dynamic mechanic
 al loadings. It is well known that in a blast scenario\, the high magnitud
 es of pressure and the rate of deformation experienced by the cells lead t
 o acute damage that contribute to a wide range of deleterious effects on c
 ellular functionalities and sometimes fatal outcomes on viability. Few res
 earchers\, however\, have addressed the problem of how cells and their com
 ponents respond to stress waves at pressure and strain rate approaching bl
 ast injury conditions. The objectives of this study are to introduce and v
 alidate a novel in vitro platform compatible with living cells to investig
 ate the effects of high-pressure stress waves across a wide range of strai
 n rates. Specifically\, an hermetically sealed sample holder was designed 
 to hold a 3D tissue construct during mechanical loading on three different
  mechanical loading devices. The sample holder was investigated as regards
  its abilities to fulfil both physical and biological requirements needed 
 to ensure the transmission of the stress pulse through the entire system w
 hilst keeping the biological material free of contamination. Multiaxial co
 mpression single pulse loading of periosteum stem cells embedded in hydrog
 el scaffold was performed at different magnitudes of stress under quasi-st
 atic\, intermediate and high rate of loading. Post-pressurisation viabilit
 y assay and sub-cellular components microscopy were performed in order to 
 examine and correlate the cellular response after mechanical insult to the
  input mechanical loadings.
LOCATION:Mott Seminar Room\, Cavendish Laboratory
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