BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Quantitative approaches to unravel the molecular mechanisms of cla
 thrin-mediated endocytosis - Julien Berro (Yale University)
DTSTART:20160623T130000Z
DTEND:20160623T134500Z
UID:TALK66555@talks.cam.ac.uk
CONTACT:INI IT
DESCRIPTION:Eukaryotic cells ubiquitously use clathrin-mediated endocytosi
 s to  internalize nutrients\, receptors and recycle plasma membrane. Defec
 ts in  endocytosis are implicated in multiple diseases such as cancer\, ne
 uropathies\,  metabolic syndromes\, and the endocytic machinery can be hij
 acked by some  pathogens to infect cells. During clathrin-mediated endocyt
 osis\, the endocytic  machinery shapes a ~50-nm diameter vesicle from the 
 flat plasma membrane in less  than 20 seconds. When membrane tension is hi
 gh\, a dynamic actin cytoskeleton is  necessary for endocytosis to proceed
 . Despite intensive studies on most of the  endocytic proteins\, it remain
 s unknown how the actin network produces the forces  necessary to deform t
 he plasma membrane during endocytosis. <br> <span><br>In this talk\, we wi
 ll show how the development of new quantitative methods  can be key to unr
 avel the molecular mechanisms of complex biological processes  such as end
 ocytosis. We will focus on new methods to measure the temporal  evolution 
 of a) the number of molecules of endocytic proteins\, b) their  residence 
 times in the endocytic structure\, c) the nanometer-scale deformations  of
  the endocytic structure\, and d) methods to increase the quality and the 
  temporal resolution of noisy datasets. We will also show how these data a
 re  invaluable to constrain mathematical models that we have developed to 
 test  hypotheses and make experimentally testable predictions.&nbsp\;</spa
 n>
LOCATION:Seminar Room 1\, Newton Institute
END:VEVENT
END:VCALENDAR