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SUMMARY:Nuclear dynamics of CSL\, the transcription factor in the Notch pa
 thway - Maria Gomez Lamarca (Sarah Bray lab\, PDN)
DTSTART:20160921T163000Z
DTEND:20160921T183000Z
UID:TALK66911@talks.cam.ac.uk
CONTACT:Clara Sidor
DESCRIPTION:The Notch pathway functions in diverse developmental processes
  as well as contributing to human diseases. In each context it regulates d
 ifferent sets of target genes. We are interested in the fundamental mechan
 isms involved in making genes Notch responsive.  Following activation\, th
 e Notch intracellular domain (NICD) is released and enters the nucleus whe
 re it forms a complex with the DNA-binding transcription factor\, CSL (CBF
 1\, Suppressor of Hairless\, Lag-1). The activity of this complex needs to
  be sensitive to the levels of Notch activity.  CSL occupancy\, measured b
 y chromatin immunoprecipitation\, is increased following Notch activation\
 , suggesting that NICD stimulates CSL movement and/or binding kinetics. Th
 ese observations inspired us to investigate CSL dynamics in the nucleus us
 ing live imaging techniques. The results reveal that\, in Notch-off cells\
 , only a small fraction of CSL is bound and that it has short residency ti
 mes. While the general properties of CSL are unchanged in Notch-on cells\,
  increased levels of CSL are detected at specific loci\, reflecting a loca
 l increase in residency that is directly dependant on interactions with NI
 CD. These changes in the binding dynamics suggest new models to explain ho
 w the core transcription complex responds to NICD/pathway activity. \n
LOCATION:Gurdon Institute Tea Room
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