BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Biotechnological Exploitation of Chlamydomonas reinhardtii - Doris
  Gangl\, Smith group
DTSTART:20161103T130000Z
DTEND:20161103T133000Z
UID:TALK67641@talks.cam.ac.uk
CONTACT:42122
DESCRIPTION:Microalgae have become increasingly important in the biotech s
 ector and are currently exploited for their natural products. In recent ye
 ars efforts have also been directed towards establishing them as productio
 n platforms for recombinant proteins. I set out to investigate the feasibi
 lity of Chlamydomonas reinhardtii as a production platform for high-value 
 products expressed in the chloroplast of the alga. A cytochrome P450 was i
 ntroduced into the chloroplast of C. reinhardtii as a proof-of-concept stu
 dy. The model enzyme CYP79A1 was successfully targeted into the chloroplas
 t membranes and was found to be active. A bifunctional diterpene synthase\
 , TPS4\, was also expressed in the chloroplast of C. reinhardtii. TPS4 cou
 ld be purified to homogeneity and is the largest enzyme expressed in the c
 hloroplast to date. The two transgenic strains expressing CYP79A1 and TPS4
  were investigated for their ability to withstand industrial growth condit
 ions. The cell wall deficient strains were successfully cultivated in 100 
 L photobioreactors using a mixotrophic growth regime. They reached dry wei
 ghts of 0.3 g/L and the expression of CYP79A1 and TPS4 was detected over t
 he entire growth period. Taken together these data suggest that C. reinhar
 dtii could be an attractive platform for recombinant protein production. T
 wo enzymes with biotechnological relevance were expressed in the chloropla
 st of the alga and the transgenic cell wall deficient strains were success
 fully cultivated on a semi-industrial scale.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
END:VEVENT
END:VCALENDAR