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SUMMARY:Ribosome profiling and virus infection - Dr Andrew Firth  Division
  of Virology ( University of Cambridge\, UK)
DTSTART:20170201T140000Z
DTEND:20170201T150000Z
UID:TALK68311@talks.cam.ac.uk
CONTACT:Emily Boyd
DESCRIPTION:While standard RNA-Seq experiments can be used to monitor the 
 abundance of every messenger RNA (mRNA) species in a cell\, this is only a
 n inaccurate proxy for actual levels of protein synthesis\, since differen
 t mRNAs can have strikingly different translation efficiencies. Further\, 
 mRNA translation efficiency can vary in response to environmental and deve
 lopmental stimuli. In contrast\, Ribosome Profiling (Ribo-Seq) provides a 
 direct measurement of protein synthesis. Ribosome profiling relies on the 
 fact that a translating ribosome protects ~30 nucleotides of mRNA. An RNA 
 nuclease is used to digest unprotected RNA\, the 30-nt ribosome-protected 
 fragments (RPFs) are purified for deep sequencing\, and the sequenced frag
 ments are mapped back to the transcriptome\, to give a global snapshot of 
 protein synthesis in the cell. Ribosome profiling has proven to be increas
 ingly valuable in studies of the translation process\, for example\, in th
 e discovery of novel translated open reading frames (ORFs)\, determination
  of elongation rates\, and identification of sites of ribosome pausing. It
  also has broad application in the analysis of global protein synthesis an
 d has been exploited in studies of infectious diseases\, cell growth\, dif
 ferentiation and development\, mitochondrial gene expression\, and cell st
 ress. In collaboration with the Brierley lab (http://www.path.cam.ac.uk/di
 rectory/ian-brierley) we have been applying Ribo-Seq and parallel RNA-seq 
 to a variety of virus species to study the kinetics of virus gene expressi
 on\, unusual transcriptional and translational mechanisms employed by viru
 ses\, and host responses to virus infection at both the transcriptional an
 d translational levels. 
LOCATION:MR4\, Centre for Mathematical Sciences\, Wilberforce Road\, Cambr
 idge
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