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SUMMARY:Biological origami: tissue remodeling in insect embryos - Kristen 
 Panfilio (University of Cologne)
DTSTART:20161102T130000Z
DTEND:20161102T140000Z
UID:TALK68701@talks.cam.ac.uk
CONTACT:Julius Bier Kirkegaard
DESCRIPTION:Early in development a protective fluid-filled sac forms aroun
 d an embryo. In humans\, this sac bursts during birth\, but the sac surrou
 nding insect embryos ruptures long before these animals begin to emerge fr
 om their eggs. The sac that surrounds insect embryos has two layers: an in
 ner layer called the amnion\, and a tough outer layer called the serosa. U
 nlike passive rupture of the human chorioamnion at birth\, the insect extr
 aembryonic (EE) tissues – the amnion and serosa – actively rupture and
  withdraw in late embryogenesis. Withdrawal is essential for development a
 nd has been a morphogenetic puzzle. Here\, we use new fluorescent transgen
 ic lines in the beetle Tribolium castaneum to show that the EE tissues dyn
 amically form a basal-basal epithelial bilayer\, contradicting the previou
 s hypothesis of EE intercalation. We find that the EE tissues repeatedly d
 etach and reattach throughout development and have distinct roles. Quantit
 ative live imaging analyses show that the amnion initiates EE rupture in a
  specialized anterior-ventral cap. RNAi phenotypes demonstrate that the se
 rosa contracts autonomously. Thus\, apposition in a bilayer enables the am
 nion as 'initiator' to coordinate with the serosa as 'driver' to achieve w
 ithdrawal. This EE strategy may reflect evolutionary changes within the ho
 lometabolous insects and serves as a model to study interactions between d
 eveloping epithelia.
LOCATION:MR11\, Centre for Mathematical Sciences\, Wilberforce Road\, Camb
 ridge
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