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SUMMARY:Detection of phage φ1207.3 in Streptococcus pneumoniae by immunoa
 ssays targeting  the major capsid protein  - Dr. Francesco Santoro\, Unive
 rsity of Siena\, LAMMB – Dept. of Medical Biotechnologies\, Siena\, Ital
 y
DTSTART:20170117T083000Z
DTEND:20170117T090000Z
UID:TALK69675@talks.cam.ac.uk
CONTACT:Ilana Spilka
DESCRIPTION:Dr. Francesco Santoro\, University of Siena\, LAMMB - Dept of 
 Medical Biotechnologies\, Siena\, Italy\nΦ1207.3 is a prophage found in a
  clinical strain of Streptococcus pyogenes which carries macrolide resista
 nce genes. We previously transferred the phage in Streptococcus pneumoniae
  and showed that it is able to produce mature and complete phage particles
 . We evaluated the presence of phage particles associated to the host in a
  bacterial culture of S. pneumoniae using polyclonal antibodies directed a
 gainst the phage major capsid protein. Φ1207.3 major capsid protein was d
 etected both inside and on the surface of the pneumococcus by western-blot
 \, flow cytometry\, immunofluorescence and immuno TEM.\n
LOCATION:Online
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