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SUMMARY:Label-free detection mechanism for carbon nanoparticles using non-
 linear optical excitation - Dr. Christian Steuwe\, Centre for Surface Chem
 istry and Catalysis\, University of Leuven
DTSTART:20170113T123000Z
DTEND:20170113T133000Z
UID:TALK70015@talks.cam.ac.uk
CONTACT:Sharon Connor
DESCRIPTION:While adverse health effects of particulate matter exposure ar
 e a generally accepted concern\, locating and tracking these nanometer siz
 ed particles is not straight forward. Measurements1-3 in polluted air such
  as absorption photometry and laser induced incandescence (LII) have so fa
 r been used to determine particle concentrations\, alternatively labeling 
 methods4-6 have been applied in epidemiological and toxicology research su
 ch as the technetium-99-m radionuclide marker. In this paper we present a 
 direct\, label-free optical contrast mechanism to detect carbon nanopartic
 les in fluids and cells7. The mechanism is non-incandescence related and d
 emonstrated with dry and suspended carbon black particles (CB)\, a widely 
 used model compound for soot8\, 9\, under illumination with femtosecond pu
 lsed near-infrared light (780 nm - 950 nm\, 150 fs\, 80 MHz). Furthermore\
 , a mechanism  based on pump-probe detection\, directly interrogating the 
 strong absorption of carbon black particles will be introduced. Picosecond
  pulsed light with various wavelengths can be applied\, as demonstrated he
 re with near-infrared light (780 nm - 1064 nm\, 7ps\, 80 MHz).\n\nFour dif
 ferent\, strongly absorbing CB species with diameters ranging from 13 to 5
 00 nm have been scrutinized\, all showing similar detection possibilities.
  Consequently\, the described WL emission and pump probe analysis allow op
 tical detection and unequivocal localization of CB particles in fluids and
  in cellular environments. The experiments are performed on a typical mult
 iphoton laser-scanning microscopy platform\, a system commonly available i
 n research laboratories.
LOCATION:Wolfson Lecture Theatre\,  Department of Chemistry
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