BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:"\;Investigating the mechanisms of regulatory T-cell different iation in vivo by novel Fluorescent Timer reporters"\; - Dr Masahiro Ono\; Department of Life Sciences\, Imperial College London DTSTART:20170220T160000Z DTEND:20170220T170000Z UID:TALK70261@talks.cam.ac.uk CONTACT:Bobbie Claxton DESCRIPTION:Foxp3+ regulatory T-cells (Treg) are considered to mainly diff erentiate in the thymus and protect against autoimmunity. However\, the ph ysiological significance of peripheral Treg differentiation is still uncle ar\, due to a lack of methods to identify newly formed cells in vivo. In a ddition\, the mechanism of Treg generation in the thymus is still obscure\ , because of difficulties in defining temporal sequences of developmental events in vivo.\n\nIn order to solve these problems\, recently we have est ablished a ground-breaking approach using Fluorescent Timer protein that s pontaneously and irreversibly changes its emission spectrum. We have devel oped novel Timer reporters using key genes for T-cell functions\, and vali dated the new reporter systems by in silico\, in vitro\, and in vivo exper iments. By developing Foxp3Timer reporter\, we reveal the mechanisms of dy namic Treg differentiation processes in the thymus and in the periphery. I n addition\, by developing a novel Timer reporter for a TCR-downstream imm ediate early gene\, we establish a TCR-responsive Timer reporter system\, and thereby reveal temporal sequences of T-cell differentiation events upo n TCR signalling in vivo.\n LOCATION:Babraham - The Brian Heap Seminar Room END:VEVENT END:VCALENDAR