BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:“Editing the genome of human induced pluripotent stem cells” -
  Dr Andrew Bassett\; Head of Research (Cellular Operations) Wellcome Trust
  Sanger Institute
DTSTART:20170221T130000Z
DTEND:20170221T140000Z
UID:TALK70869@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:The advent of human induced pluripotent stem cell (hiPSC) tech
 nology has provided a unique opportunity to establish cellular models of d
 isease from individual patients\, to study the effects of the underlying g
 enetic aberrations upon multiple different cell types\, and understand the
  underlying molecular and cellular phenotypes. Combining this with recent 
 advances in genome editing techniques such as the clustered regularly inte
 rspaced short palindromic repeat (CRISPR) system has provided an ability t
 o repair putative causative alleles in patient lines\, or introduce diseas
 e alleles into a healthy “WT” cell line to generate isogenic cell pair
 s that differ in a single genetic change. The simplicity of this technolog
 y also enables screening methods to be employed to identify the true causa
 tive lesion\, which is often impossible to ascertain from human genetic st
 udies alone\, and frequently sit within the non-coding genome. I will desc
 ribe our current technologies that allow highly efficient introduction of 
 a variety of genetic changes in iPSCs and discuss the uses of this technol
 ogy for in understanding and annotating functional genomic elements.
LOCATION:Babraham - The Brian Heap Seminar Room
END:VEVENT
END:VCALENDAR