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SUMMARY:Recent advances in the molecular diagnosis of canine and feline ly
 mphoma - Dr Sabine Hammer (Molecular Geneticist)\,  and Dr Barbara  Rütge
 n (Clinical Pathologist) Dept of Pathobiology\, University of Veterinary M
 edicine\, Vienna
DTSTART:20170517T150000Z
DTEND:20170517T160000Z
UID:TALK71016@talks.cam.ac.uk
CONTACT:Fiona Roby
DESCRIPTION:Clonality is a fundamental property of neoplasia and the evalu
 ation of the clonal nature of a lymphocyte population within tissue sample
 s is commonly used as an adjunctive diagnostic  procedure in humans. In re
 cent years\, molecular techniques have become available for the diagnosis 
 of canine\, feline and equine lymphoproliferative disorders. Polymerase ch
 ain reaction (PCR) for antigen receptor rearrangement is a method used for
  the detection of clonal lymphocyte populations based on the assessment of
  diversity within the complementary determining region 3 of the immunoglob
 ulin heavy chain variable region genes and the T cell receptor gamma genes
 . It represents a sensitive and flexible PCR assay system for the analysis
  of various source materials e.g. fine needle aspirates\, whole blood\, bo
 ne marrow\, cerebrospinal fluid also allowing retrospective studies based 
 on formaline fixed paraffin embedded material. In addition to the clinical
 \, cytological\, histological and immunophenotyping data of a single patie
 nt\, this technique is a suitable adjunctive method in distinguishing ambi
 guous cases in which morphology cannot distinguish between a reactive or n
 eoplastic lymphocytic population. However every molecular method transferr
 ed into diagnostic routine must be standardised\, validated and controlled
  to ensure high quality results that have far reaching consequences for th
 e patient. This lecture will give an introduction to the method of clonali
 ty testing in dogs and cats\, will discuss indication\, preanalytic consid
 erations\, recommendations for assay performance and interpretation\, sens
 itivity/specificity and will present patient case examples showing the rou
 tine practical application.\n
LOCATION:Lecture Theatre 2\, Department of Veterinary Medicine
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