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SUMMARY:Studying long-distance enhancers of Sox9 in the developing mouse g
 onad - Dr Nitzan Gonen\; Crick Institute\, London
DTSTART:20170713T120000Z
DTEND:20170713T130000Z
UID:TALK73040@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:SOX9 is a transcription factor known to initiate Sertoli cell 
 differentiation and testis development and is expressed throughout life in
  Sertoli cells. Expression of SOX9 in the early XX gonad can cause female-
 to-male sex reversal\, whereas loss of function mutations of Sox9 in XY an
 imals can cause male-to-female sex reversal. As Sox9 is widely expressed i
 n many tissues and cell types\, and it harbour a very big and complex regu
 latory region of 2MB (also called the “2MB desert”) we initiated an at
 tempt to discover the enhancers that regulate Sox9 expression specifically
  in the testis. A 3.2 kb enhancer located 13 kb upstream of Sox9 start sit
 e was discovered and termed TES (Testis-specific enhancer of Sox9). Althou
 gh able to completely mimic Sox9 expression pattern in the testis\; target
 ed deletion of this enhancer using the CRISPR/Cas9 technique failed to ind
 uce sex reverse and resulted in 55% reduction in Sox9 mRNA levels in the t
 estis. This indicates that although being important\, TES may be not the s
 ole enhancer regulating Sox9 expression in the testis. To address that\, w
 e performed DNaseI hypersensitivity assay as well as ATAC-seq and H3K27ac 
 ChIP-seq in Sertoli cells to identify potential active enhancers in the up
 stream 2MB gene desert of Sox9. Progress in identifying those enhancers an
 d studying their functional roles will be presented and discussed. 
LOCATION:Babraham - The Brian Heap Seminar Room
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