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SUMMARY:Pub tutorial: Real-time control of a genetic toggle switch - Jean-
 Baptiste Lugagne\, Laboratoire Matière et Systèmes Complexes (MSC)\, Uni
 versité Paris Diderot &amp\; CNRS
DTSTART:20170719T170000Z
DTEND:20170719T190000Z
UID:TALK73651@talks.cam.ac.uk
CONTACT:Dr David Jörg
DESCRIPTION:Recent progresses in microfluidics\, synthetic biology\, and m
 icroscopy automation now make it possible to control gene expression exter
 nally and in real time. Among the challenges facing the field of external 
 real­time control of gene expression is the control of intricate\, multis
 table gene regulation networks as well as the control of several target ge
 nes at the same time. To advance the domain in this direction\, I studied 
 the controllability of a simple bistable two-­genes network\, the so­-ca
 lled genetic toggle switch\, in the vicinity of its unstable equilibrium p
 oint for extended periods of time. In this talk\, I will present the devel
 opment of a custom control platform for external control of gene expressio
 n at the single­-cell level as well as a bacterial cellular chassis and a
  library of toggle switch genetic circuits. This platform is used to drive
  and maintain the genetic toggle switch in its region of instability with 
 both closed-­loop and open­-loop strategies\, for single cells and for p
 opulations of cells. Finally\, both experimental and theoretical results d
 emonstrate the emergence of new regimes of stability in gene regulation ne
 tworks when submitted to oscillating chemical environments because of dyna
 mic stabilization phenomenons\, and can bring new insights to the study of
  cellular decision­-making.
LOCATION:Panton Arms\, 43 Panton St\, Cambridge CB2 1HL
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