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SUMMARY:&quot\;Live cell biochemistry by light&quot\; - Dr Alessandro Espo
 sito\; MRC Cancer Unit\, University of Cambridge
DTSTART:20171006T120000Z
DTEND:20171006T130000Z
UID:TALK76502@talks.cam.ac.uk
CONTACT:Bobbie Claxton
DESCRIPTION:Many forms of cancer begin with oncogenic events\, which\, in 
 normal cells\, elicit growth cessation or cell death rather than transform
 ation\, due to the activation of tumour suppressive networks. We are inter
 ested in dissecting the signaling pathways that lead from oncogenic events
  to tumour suppressive mechanisms\, and in particular\, how cells integrat
 e extracellular and intracellular signals to preserve tissue-\, cell- and 
 genome homeostasis in the face of oncogenic challenge.\nHowever\, we recog
 nize that state of the art technologies do not permit to characterize a nu
 mber of biochemical reactions in the living cells thereby limiting our cap
 abilities to establish causal dependencies between the spatiotemporal dyna
 mics of biochemical networks and cellular decisions. Therefore\, we devote
 d significant efforts to develop novel fluorescence-based assays with the 
 aim to enable complex biochemical measurements in single living cells. \nI
 n this talk\, I will review state-of-the-art biochemical imaging technique
 s (e.g.\, translocation sensors for phosphorylation\, FRET-based sensors a
 nd FLIM) and techniques for the light-dependent induction of biochemical e
 vents. Furthermore\, I will demonstrate our latest developments of fast no
 n-invasive techniques to simultaneously measure several biochemical reacti
 ons within the living cells with high spatio-temporal resolution and the u
 se of optogenetics in cell biology and cancer biology (e.g.\, fast activat
 ion of oncogenic signalling) in particular.\nThe possibility to interrogat
 e signal transduction pathways non-invasively with the highest spatiotempo
 ral and biochemical resolutions will enable us to elucidate mechanisms und
 erlying oncogenesis and their cellular heterogeneity. 
LOCATION:Babraham - The Brian Heap Seminar Room
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