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SUMMARY:Alternative splicing in the human transcriptome: Functional and st
 ructural influence on proteins - Dr. Kei Yura\, Quantum Bioinformatics Tea
 m\, Japan Atomic Energy Agency\, Kyoto\, Japan
DTSTART:20070912T120000Z
DTEND:20070912T130000Z
UID:TALK8004@talks.cam.ac.uk
CONTACT:Gos Micklem
DESCRIPTION:Alternative splicing is a molecular mechanism that produces mu
 ltiple proteins from a single gene\, and is thought to produce variety in 
 proteins translated from a limited number of genes. Here we analyzed how a
 lternative splicing produced variety in protein structure and function\, b
 y using human full-length cDNAs on the assumption that all of the alternat
 ively spliced mRNAs were translated to proteins. We found that the length 
 of alternatively spliced amino acid sequences\, in most cases\, fell into 
 a size shorter than that of average protein domain. We evaluated comprehen
 sively the presumptive three-dimensional structures of the alternatively s
 pliced products to assess the impact of alternative splicing on gene funct
 ion. We found that more than half of the products encoded proteins which w
 ere involved in signal transduction\, transcription and translation\, and 
 more than half of alternatively spliced regions comprised interaction site
 s between proteins and their binding partners\, including substrates\, DNA
 /RNA\, and other proteins. Intriguingly\, 67% of the alternatively spliced
  isoforms showed significant alterations to regions of the protein structu
 ral core\, which likely resulted in large conformational change. Based on 
 those findings\, we speculate that there are a large number of cases that 
 alternative splicing modulates protein networks through significant altera
 tion in protein conformation.
LOCATION:Bateson Room\, Department of Genetics
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