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SUMMARY:Using the auxin-inducible degron in yeast to understand the splice
 osome cycle - Gonzalo Mendoza\, Smith Lab
DTSTART:20180118T130000Z
DTEND:20180118T133000Z
UID:TALK82681@talks.cam.ac.uk
CONTACT:Pallavi Singh
DESCRIPTION:Pre-mRNA splicing is essential for eukaryotic cells and is cat
 alyzed by a very large RNA/protein particle called the spliceosome. During
  my PhD\, my initial research aim was to identify proteins potentially inv
 olved in linking splicing with transcription. To this end\, I used the aux
 in-inducible degron technique to quickly deplete proteins of interest\, an
 d chromatin immunoprecipitation to measure in vivo assembly of the spliceo
 some. I discovered that knocking-down splicing proteins can quickly lead t
 o strong secondary effects that can mask the primary consequence of the de
 pletion. I will explain where these secondary effects are coming from\, su
 ggest one way to avoid them and discuss the implications of this finding. 
 Towards the end of my talk\, I will present the plans I have of applying p
 re-mRNA splicing techniques to engineer the thiamine-responsive riboswitch
  of Chlamydomonas reinhardtii. My goal is to create novel molecular tools 
 to control transgene expression in plants.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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