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SUMMARY:CANCELLED-Open tools in Marchantia for plant bioengineering work a
 nd as a platform for elucidating morphogenesis - Susana Sauret-Gueto\, Has
 eloff Lab
DTSTART:20180301T130000Z
DTEND:20180301T133000Z
UID:TALK82711@talks.cam.ac.uk
CONTACT:Pallavi Singh
DESCRIPTION:At OpenPlant we are establishing Marchantia as a testbed for p
 lant synthetic biology. The relative simplicity of genetic networks in Mar
 chantia\, combined with the growing set of genetic manipulation\, culture 
 and microscopy techniques\, are set to make this primitive plant a major n
 ew system for analysis and engineering.\n\nI will update on the open tools
  we are generating in Prof. Haseloff lab\, and how we aim at applying them
  for elucidating and engineering morphogenesis. \n\nWe are setting up stan
 dardised practices for DNA assembly\, compiling a Marchantia DNA toolkit a
 nd establishing registries to facilitate standardisation and sharing. The 
 constructs are compatible with the OpenMTA\, and thus suitable for open di
 stribution. The DNA toolkit includes parts for selection of successful tra
 nsformants\, quantitative imaging of multispectral markers and targeted mu
 tagenesis with CRISPR/Cas9\, and aims at maximising efficiency and reprodu
 cibility of Marchantia workflows.\n\nWe have also generated a collection o
 f proximal promoters of all Marchantia transcription factors (TFs)\, to sc
 reen for tissue specific expression patterns. Another source of tissue-spe
 cific lines comes from the screening of the enhancer trap lines developed 
 in the lab.  We are especially interested in lines that tag specific cell 
 types and reveal developmental transitions during air chamber development.
  To help elucidating air chamber morphogenesis we aim at doing a targeted 
 high-throughput mutagenesis screen of Marchantia TFs with a library of gRN
 As.\n\nThe synthetic biology\, Marchantia and wider plant science communit
 ies are coming together around OpenPlant and being pivotal in bringing its
  goals forward.\n
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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