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SUMMARY:Open tools in Marchantia for plant bioengineering work and as a pl
 atform for elucidating morphogenesis - Susana Sauret-Gueto\, Haseloff Lab
DTSTART:20180503T120000Z
DTEND:20180503T123000Z
UID:TALK82771@talks.cam.ac.uk
CONTACT:Pallavi Singh
DESCRIPTION:\nAt OpenPlant we are establishing Marchantia as a testbed for
  plant synthetic biology. The relative simplicity of genetic networks in M
 archantia\, combined with the growing set of genetic manipulation\, cultur
 e and microscopy techniques\, are set to make this primitive plant a major
  new system for analysis and engineering.\nI will update on the open tools
  we are generating in Prof. Haseloff lab\, and how we aim at applying them
  for elucidating and engineering morphogenesis.\n\nWe are setting up stand
 ardised practices for DNA assembly\, compiling a Marchantia DNA toolkit an
 d establishing registries to facilitate standardisation and sharing. The c
 onstructs are compatible with the OpenMTA\, and thus suitable for open dis
 tribution. The DNA toolkit includes parts for selection of successful tran
 sformants\, quantitative imaging of multispectral markers and targeted mut
 agenesis with CRISPR/Cas9\, and aims at maximising efficiency and reproduc
 ibility of Marchantia workflows.\n\nWe have also generated a collection of
  proximal promoters of all Marchantia transcription factors (TFs)\, to scr
 een for tissue specific expression patterns. Another source of tissue-spec
 ific lines comes from the screening of the enhancer trap lines developed i
 n the lab.  We are especially interested in lines that tag specific cell t
 ypes and reveal developmental transitions during air chamber development. 
 To help elucidating air chamber morphogenesis we aim at doing a targeted h
 igh-throughput mutagenesis screen of Marchantia TFs with a library of gRNA
 s. The synthetic biology\, Marchantia and wider plant science communities 
 are coming together around OpenPlant and being pivotal in bringing its goa
 ls forward. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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